Research Article
Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs
Table 1
Pairs of primers designed for the analysis of the expression of target genes for Leishmania spp. and dog using PCR assays.
| | GeneDB ID | Gene-target | Primers foward/reverse | Product size (pb) |
| | Leishmania spp. genes | LinJ.10.0501 | Metallo protease: gp63, leishmanolysin | 5 GGGTAGGGGCGTTCTGC 3
5 CCCGGCCTATTTTACACCAACC 3 | 202 | | LinJ.10.0510 | | LbrM.10.0610 | | LbrM.10.0590 | | LbrM.08.0810 | Cysteine protease: cathepsin L-like protease | 5 GGGTAGGGGCGTTCTGC 3
5 CCCGGCCTATTTTACACCAACC 3 | 227 | | LbrM.08.0820 | | LbrM.08.0830 | | LinJ.08.0960 | | M94088 | Kinetoplast minicircle 3 (kDNA3) | 5 CTGATCCACTGTTTTCTCCCCA 3
5 AAAGTGCCCGTGAGTACAGG 3 | 120 |
| | Canis lupus familiaris | LOC106557476 | α-Tubulin | 5 GGGTAGGGGCGTTCTGC 3
5 CCCGGCCTATTTTACACCAACC 3 | 160 | | XM_003124280.5 | β-Actin | 5 CTGATCCACTGTTTTCTCCCCA 3
5 AAAGTGCCCGTGAGTACAGG 3 | 87 |
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