Tanshinone IIA Alleviates CCL2-Induced Leaning memory and Cognition Impairment in Rats: A Potential Therapeutic Approach for HIV-Associated Neurocognitive Disorder

Figure 9

Effects of Tanshinone IIA on caspase-3, caspase-8, and caspase-9 mRNA expression in the hippocampus of CCL2 treated rats. Quantitative real-time PCR analysis of messenger (m) RNA levels. Relative expression of (a) caspase-3 mRNA, (b) caspase-8 mRNA, and (c) caspase-9 mRNA. qPCR analysis showed increased expression of caspase-3, caspase-8, and caspase-9 mRNA in the hippocampus of model rats. Caspase-3, caspase-8, and caspase-9 mRNA expression in the Tanshinone IIA treatment groups (Tan IIA(L): 25 mg/kg, Tan IIA(M): 50 mg/kg; Tan IIA(H): 75 mg/kg) decreased compared to the CCL2 group (, ) but showed no statistically significant difference between the model and Tanshinone IIA treatment groups on caspase-9 mRNA expression (). The experiments were repeated three times independently. All data are expressed as mean ± SEM (n = 3, , versus model; , versus sham).

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