IL-4 Alleviates Ischaemia-Reperfusion Injury by Inducing Kupffer Cells M2 Polarization via STAT6-JMJD3 Pathway after Rat Liver Transplantation

Figure 4

IL-4 induced KCs M2 polarization was dependent on the STAT6-JMJD3 pathway in vitro. KCs were isolated from untreated livers, and IL-4-treated KCs were pretreated with siRNA-JMJD3 or the STAT6 inhibitor As1517499. (a, b) KCs were incubated for the indicated times or with different concentrations of IL-4 for 12 h; then the protein expression level of JMJD3 was detected by Western blot (n = 3/group). (c, d) The ratio of F4/80⁺CD206⁺ KCs was measured by flow cytometry, and the expression of Arg-1 and JMJD3 was analysed by Western blot (n = 3/group). (e) The protein expression levels of JMJD3, p-STAT6, and H3K27me3 were detected by Western blot. GAPDH served as an internal control and was used for normalization (n = 3/group). Data are shown as mean ± SD, vs. the control group, vs. the IL-4 group, vs. the IL-4 + As1517499 group.

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