ATP-Sensitive Potassium Channels Mediate the Cardioprotective Effect of Panax notoginseng Saponins against Myocardial Ischaemia–Reperfusion Injury and Inflammatory Reaction

Figure 4

PNS pretreatment improved the expression and opening of K_ATP in rats with myocardial reperfusion injury. (a) Subunits of K_ATP in rats with myocardial reperfusion injury were measured using western blotting. (b–e) Quantitative analysis results for Kir6.1, Kir6.2, SUR1, and SUR2, respectively. (f–i) Quantitative analysis results for mRNA of Kir6.1, Kir6.2, SUR1, and SUR2 from real-time PCR, respectively. (j) Representative traces of whole-cell currents at voltage-clamp pulses at voltages ranging from −80 to 80 mV. (k) Pretreatment with PNS (50, 100 mg/L) on left ventricular cardiomyocytes at 0 mV after I/R (). vs. Sham, # vs. I/R, ^▲ vs. I/R+PNS.

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