ATP-Sensitive Potassium Channels Mediate the Cardioprotective Effect of Panax notoginseng Saponins against Myocardial Ischaemia–Reperfusion Injury and Inflammatory Reaction

Figure 6

PNS inhibited NF-κB phosphorylation and nuclear translocation in reperfused myocardia. (a) Protein levels of p-NF-κB in cytoplasm; total NF-κB level was used as a control. (b) Quantitative analysis of the ratios of p-P65/P65. (c) Immunofluorescence results indicating changes in the expression and nuclear translocation of p65 under different treatments (p65 was labelled with red fluorescence, original magnification ×400). The yellow arrow indicates that p-NF-κB was colocalized with the nucleus. (d) Quantitative analysis of the fluorescence intensity of p65 in each group (). , # vs. I/R, ^▲ vs. I/R+PNS. The primers encoding rat TNF-α, MCP-1, Kir6.1, Kir6.2, SUR1, SUR2, and GAPDH are presented in the following table.

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