Mesenteric Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats

Figure 6

The morphological characteristics of PMVECs treated with mesenteric lymph (magnification, ×400). There was no morphologic change in PMVECs cocultivated with 10%PBS (a). When cocultured with 10% septic lymph, PMVECs showed shrinkage and detached from the bottom of the culture flask (c). In contrast, the injury of PMVECs was significant lessened when cocultured with 10% normal lymph (b). The cell viability of PMVECs was decreased by treatment with septic lymph for 24 h compared with that in the lymph group as measured by the MTT assay (d). TNF-α, IL-1β, and IL-6 mRNA expressions were significantly increased in PMVECs cocultivated with septic lymph compared with that in the normal lymph group (e). Results were analyzed using two-way repeated measures ANOVA with protected posthoc testing. vs.10% PBS group and 10% normal lymph.

(a)

(b)

(c)

(d)

(e)