Downregulation of CRABP2 Inhibit the Tumorigenesis of Hepatocellular Carcinoma In Vivo and In Vitro

Figure 3

Effects of CRABP2 on cell proliferation, metastasis, and apoptosis. (a, b) CCK-8 assays were carried out to assess the cell viability of HpeG2 and Hun7 cells after transfection with CRABP2-shRNAs. . (c, d) Cell proliferation of HpeG2 and Hun7 cells was determined by EDU staining, the positive cell rate was analyzed by Image-Pro Plus software, . . (e, f) Migration and invasion of HpeG2 and Hun7 cells was assessed by Transwell assay (200x); the relative cell number rate was analyzed by Image-Pro Plus software. . (g, h) CRABP2-shRNA transfection increased HpeG2 and Hun7 cell apoptosis in flow cytometry. . One-way ANOVA was used in multigroup comparison. All data are presented as . , vs. NC-shRNA.

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