Downregulated miR-204 Promotes Skeletal Muscle Regeneration

Figure 2

miR-204 regulates C2C12 myoblast proliferation through Pax7. (a) The binding site of miR-204 to Pax7. (b) The Pax7 3’UTR with wild-type (wt) or mutant type (mut) were inserted into psiCHECK™-2 vectors and cotransfected with miR-204 mimic or negative control into HeLa cells, respectively. . (c–e) The expression of miR-204 (c), Pax7 (d), and cell division cycle genes (CCNB (Cyclin B), CCND (Cyclin D), CCNE (Cyclin E), CDK4 (Cyclin-dependent kinase 4)) in the C2C12 myoblasts after transfection with miR-204 mimic or inhibitor. . (f) Cell counts measured using the Cell Count Kit 8 (CCK-8) method. . (g, h) The EdU assay was carried out after a 48h transfection. C2C12 myoblasts undergoing DNA replication were stained by EdU (red), and cell nuclei were stained with Hoechst (blue). Scale , . Data are presented as . , .

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