Downregulated miR-204 Promotes Skeletal Muscle Regeneration

Figure 3

miR-204 regulates C2C12 myoblast migration through IGF1 (insulin-like growth factor 1). (a) The binding site of miR-204 to IGF1. (b) The IGF1 3’UTR with wild-type (wt) or mutant type (mut) were inserted into the psiCHECK™-2 vectors and cotransfected with miR-204 mimic or negative control into HeLa cells, respectively. . (c) The expression of Pax3 (Paired box 3) and IGF1 in the C2C12 myoblasts after transfection with miR-204 mimic or inhibitor. . (d, e) Scratch test pictures of cell monolayers at time 0, 12 hours, and 24 hours following initiation of scratch defect on C2C12 myoblasts. Scale , . Data are presented as . , .

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