Downregulated miR-204 Promotes Skeletal Muscle Regeneration

Figure 4

miR-204 regulates C2C12 myoblasts differentiation through Mef2c (myocyte enhancer factor 2c). (a) The binding sites of miR-204 to Mef2c. (b) The Mef2c 3’UTR with wild-type (wt) or mutant type (mut) were inserted into the psiCHECK™-2 vectors and cotransfected with miR-204 mimic or negative control into HeLa cells, respectively. . (c, d) The expression of miR-204, MyoD, MyoG, Mef2c, and MyHC in the C2C12 myotubes after transfection with miR-204 mimic or inhibitor. . (e, f) Immunofluorescence of MyHC (muscle myosin heavy chain) in C2C12 myotubes. Scale , . Data are presented as . , .

(a)

(b)

(c)

(d)

(e)

(f)