Downregulated miR-204 Promotes Skeletal Muscle Regeneration

Table 1

The primers used for qRT-PCR and the synthesized RNA oligonucleotides.

Gene	Primer sequence (5-3)	TM/°C
Pax3	F-CCGGGGCAGAATTACCCAC	60.6
	R-GCCGTTGATAAATACTCCTCCG
Pax7	F-TGGGGTCTTCATCAACGGTC	60
	R-ATCGGCACAGAATCTTGGAGA
IGF1	F-GGACGGGGACTTCTGAGTCTT	60.6
	R-AGAGAGCGGGACTCCTTCTG
Mef2c	F-ATCTCTCCCTGCCTTCTACTC	60
	R-CTCCCATCGTAGGAACTGCT
MyoG	F-GCCCAGTGAATGCAACTCCCACA	58
	R-CAGCCGCGAGCAAATGATCTCCT
MyoD	F-AGACTTCTATGATGACCCGTGTT	57
	R-TCAGCGTTGGTGGTCTTGC
MyHC	F-GCGAATCGAGGCTCAGAACAA	60
	R-GTAGTTCCGCCTTCGGTCTTG
CCNB	F-AAGGTGCCTGTGTGTGAACC	61
	R-GTCAGCCCCATCATCTGCG
CCND	F-GCGTACCCTGACACCAATCTC	61
	R-CTCCTCTTCGCACTTCTGCTC
CCNE	F-GTGGCTCCGACCTTTCAGTC	60
	R-CACAGTCTTGTCAATCTTGGCA
CDK4	F-ATGGCTGCCACTCGATATGAA	60
	R-TCCTCCATTAGGAACTCTCACAC
β-Actin	F-TGGAATCCTGTGGCATC CATGAAAC	60
	R-TAAAACGCAGCTCAG TAACAGTCCG
miR-204	F-TTCCCTTTGTCATCCTATGCCT	60
	R- Uni-miR qPCR primer, included in kit (miRNA universal downstream primer, TaKaRa)
U6	F: CTCGCTTCGGCAGCACA	60
	R: AACGCTTCACGAATTTGCGT

F: forward; R: reverse.