Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots

<div>Identification of recombinant sdAbE2s by SDS-PAGE and Western blot. (a) Analysis of expression sdAbE2 with SDS-PAGE. Lane M protein MW marker; lanes 1 and 6 were the expression groups of sdAbE2-1 and sdAbE2-2 induced with IPTG. Lanes 2 and 5 were the Ni-NTA-affinity purified sdAbE2-1 and sdAbE2-2. Lanes 3 and 4 were purified products of sdAbE2-1 and sdAbE2-2 by the cleavage of fusion tag of SUMO and 6 × His tag. (b) Verification by Western blotting. Western blotting was performed using HRP-anti-His mouse monoclonal antibody and ECL substrate. Lanes 7 and 8 were the lysates of expression group and purification group of sdAbE2-1. Lanes 9 and 10 were the lysates of expression group and purification group of sdAbE2-2. Lane 11 uninduced control group.</div>

BioMed Research International

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Figure 2

Figure 2: Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots 

Figure 2 | Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots 