Analysis of Acrolein Exposure Induced Pulmonary Response in Seven Inbred Mouse Strains and Human Primary Bronchial Epithelial Cells Cultured at Air-Liquid Interface

Figure 1

Lung transcript analysis of oxidative stress markers in seven mouse strains exposed to clean air (sham) or acrolein (1 ppm, 6 hours/day, 4-5 days/week for 11 weeks): (a) glutathione peroxidase 1 (Gpx1); (b) glutathione peroxidase 3 (Gpx3); (c) superoxide dismutase 3, extracellular (Sod3); (d) heme oxygenase 1 (Hmox1); (e) nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (Nfkb1). Beta actin (Actb) served as the housekeeping control gene. Data are presented as an expression in the exposed group relative to age and strain-matched sham-exposed group (median and 25th-75th percentiles; mice/strain/stage); white bars: sham, gray bars: acrolein exposed. value < 0.05 and value < 0.01; sham vs. exposed group of each strain. ^# value < 0.05, exposed BALB/cByJ vs. other exposed strains like DBA/2J, C3H/HeJ, and A/J. ^ǂ value < 0.05 and ^ǂǂ vaue < 0.01; exposed C57BL/6J vs. exposed strains like DBA/2J, C3H/HeJ, and FVB/NJ. ⁺ value < 0.05, exposed 129S1/SvlmJ vs. exposed FVB/NJ. ^x value < 0.05, exposed C3H/HeJ vs. exposed A/J vs. FVB/NJ.

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