Analysis of Acrolein Exposure Induced Pulmonary Response in Seven Inbred Mouse Strains and Human Primary Bronchial Epithelial Cells Cultured at Air-Liquid Interface

Figure 2

Lung transcript analysis of proinflammatory markers in seven mouse strains exposed to clean air (sham) or acrolein (1 ppm, 6 hours/day, 4-5 days/week, 11 weeks): (a) chemokine (C-X-C motif) ligand 1 (Cxcl1); (b) chemokine (C-X-C motif) ligand 2 (Cxcl2); (c) interleukin-6 (IL-6); (d) interleukin-17B (IL-17B); (e) tumor necrosis factor (Tnf). Beta actin (Actb) served as the control. Data are presented as an expression in the exposed group relative to age and the strain-matched sham-exposed group (median and 25th-75th percentiles; mice/strain/stage). Mouse strains: 129S1/SvlmJ, A/J, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, and FVB/NJ; white bars: sham, gray bars: acrolein exposed. value < 0.05 and ; sham vs. exposed group of each strain. ^# and ^##; exposed BALB/cByJ vs. other exposed strains like DBA/2J, C3H/HeJ, and A/J. ^ǂ value < 0.05 and ^ǂǂ value < 0.01; exposed C57BL/6J vs. exposed strains like DBA/2J, C3H/HeJ, and FVB/NJ. ⁺ value < 0.05, exposed 129S1/SvlmJ vs. exposed strains DBA/2J, C3H/HeJ, A/J, and FVB/NJ. ^♦ value < 0.05, exposed DBA/2J vs. exposed. ^§ value < 0.05, exposed A/J vs. exposed FVB/NJ.

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