Analysis of Acrolein Exposure Induced Pulmonary Response in Seven Inbred Mouse Strains and Human Primary Bronchial Epithelial Cells Cultured at Air-Liquid Interface

Figure 3

Lung transcript analysis of tissue injury markers in seven mouse strains exposed to clean air (sham) or acrolein (1 ppm, 6 hours/day, 4-5 days/week, 11 weeks): (a) matrix metallopeptidase 9 (Mmp9); (b) tissue inhibitor of metalloproteinase 1 (Timp1). Beta actin (Actb) served as the control. Data are presented as an expression in the exposed group relative to age and the strain-matched sham-exposed group (median and 25th-75th percentiles; mice/strain/stage). Mouse strains: 129S1/SvlmJ, A/J, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, and FVB/NJ; white bars: sham; gray bars: acrolein exposed. value < 0.05, value < 0.01: sham vs. exposed group of each strain. ^# value < 0.05, exposed BALB/cByJ vs. exposed 129S1/SvlmJ, C3H/HeJ, and A/J. ⁺ value < 0.05, exposed 129S1/SvlmJ vs. exposed DBA/2J and C3H/HeJ.

(a)

(b)