Analysis of Acrolein Exposure Induced Pulmonary Response in Seven Inbred Mouse Strains and Human Primary Bronchial Epithelial Cells Cultured at Air-Liquid Interface

Figure 4

Transcript analysis of IL-17 family members (IL-17A, B, C, D, and F) in primary bronchial epithelial cells (PBEC) cultured at an air-liquid interface (ALI) condition after 24 h incubation following a 30-minute exposure to clean air (sham), 0.1 or 0.2 ppm acrolein vapor: (a) interleukin-17A (IL-17A); (b) interleukin-17B (IL-17B); (c) interleukin-17C (IL-17C); (d) interleukin-17D (IL-17D); (e) interleukin-17F (IL-17F). Beta actin (ACTB) served as the control. Data are presented as median and 25th-75th percentiles ( donors and replicates/donor). value < 0.05, value < 0.01: sham vs. exposed group; ^# value < 0.05 0.1 ppm vs. 0.2 ppm acrolein exposed. White bars: sham (clean air); light gray bars: 0.1 ppm; dark gray bars: 0.2 ppm acrolein exposed.

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