Analysis of Acrolein Exposure Induced Pulmonary Response in Seven Inbred Mouse Strains and Human Primary Bronchial Epithelial Cells Cultured at Air-Liquid Interface

Figure 5

Transcript analysis of interleukin-17 (IL-17) pathway-related markers (IL-1B, IL-22, RORA, and STAT3) in primary bronchial epithelial cells (PBEC) cultured at an air-liquid interface (ALI) condition after 24 h of incubation following a 30-minute exposure to clean air (sham), 0.1 or 0.2 ppm acrolein vapor. Transcript expression of interleukin-1 beta (IL-1B), interleukin-22 (IL-22), RAR-related orphan receptor A (RORA), and signal transducer and activator of transcription 3 (STAT3) was analyzed following 24 h of exposure. Data are presented as median and 25th-75th percentiles ( donors and replicates/donor). value < 0.05; value < 0.01; white bars: sham (clean air); light gray bars: 0.1 ppm exposed; dark gray bars: 0.2 ppm acrolein exposed.

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