The Role of Overexpressed Apolipoprotein AV in Insulin-Resistant Hepatocytes

Figure 2

Successful construction of the insulin-resistant HepG2 cells with APOAV overexpression. (a) Recombinant adenoviruses packing HEK293 cells were infected with recombinant adenoviruses with APOAV overexpression. (b) The fluorescence images of insulin-resistant HepG2 cells with APOAV overexpression. B-M: insulin-resistant HepG2 cells with APOAV overexpression; A-con: negative controls. (c) Western blot analysis for APOAV 5 expression in control and insulin-resistant HepG2 cells with APOAV overexpression. (d) Glucose uptake rate and (e) liver glycogen synthesis in normal control, insulin-resistant HepG2 cells, and insulin-resistant HepG2 cells with APOAV overexpression.

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