Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses <i>in vitro</i>

<div>Immune response by PBMC cell surface markers to polyurethane surface roughness. PBMCs were cultured on polyurethane with different surface roughness (P0-P7) for three days, and the expression of cell surface markers on T cells (lymphocytes), NK cells, and monocytes was assessed. Expression of HLA-DR and CD16 on CD3<sup>+</sup> T cells (a) HLA-DR on CD3<sup>-</sup>/CD16<sup>+</sup> natural killer cells (b) and HLA-DR, CD86, and CD163 on CD14<sup>+</sup> monocytes (c) from PBMCs cultured on polyurethane samples with increasing roughness from P0 (flat) to P7 (roughest). Data not shown for PD-1 on T cells/NK cells and CD16 on monocytes. 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Experiments included the testing of three donors, while graphs show one representative donor. TCP: tissue culture polystyrene.</div>

BioMed Research International

fig7

Figure 7

Figure 7: Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses <i>in vitro</i> 

BioMed Research International

Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro

Figure 7