Circular RNA circ-CCAC1 Facilitates Adrenocortical Carcinoma Cell Proliferation, Migration, and Invasion through Regulating the miR-514a-5p/C22orf46 Axis

Figure 3

circ-CCAC1 sponges miR-514a-5p to upregulate C22orf46 expression in ACC. (a) RT-qPCR detection of the percentage of circ-CCAC1 in the cytoplasmic and nuclear fractions of H295R cells. (b) RT-qPCR detection of the percentage of circ-CCAC1 in the cytoplasmic and nuclear fractions of SW-13 cells. (c) Venn diagram showing the number of overlapping miRNAs. (d) Relative expression levels of miR-182-5p, miR-1343-3p, miR-514a-5p, miR-3619-5p, and miR-6746-5p were detected after silencing of circ-CCAC1 in H295R cells. (e) Relative expression levels of miR-182-5p, miR-1343-3p, miR-514a-5p, miR-3619-5p, and miR-6746-5p were detected after silencing of circ-CCAC1 in SW-13 cells. (f) Relative expression of miR-514a-5p in ACC tissues and normal tissues by RT-qPCR. (g) Kaplan-Meier analysis of overall survival in ACC patients according to miR-514a-5p expression by TCGA data. (h) Relative expression of miR-514a-5p in ACC and normal cells by RT-qPCR. (i) Correlation analysis of circ-CCAC1 and miR-514a-5p expression in ACC samples. (j) Schematic illustration of circ-CCAC1-Wt/Mut luciferase reporter vectors. (k) The binding ability between circ-CCAC1 and miR-514a-5p was detected by dual-luciferase reporter assay in H295R and SW-13 cells. (l) Correlation analysis of circ-CCAC1 and C22orf46 mRNA expression in ACC samples. (m) Kaplan-Meier analysis of overall survival in ACC patients according to C22orf46 expression by TCGA data. (n) Relative expression of C22orf46 mRNA in ACC and normal cells by RT-qPCR. (o) Relative expression of C22orf46 in ACC and normal cells by Western blotting. (p) C22orf46 mRNA expression was detected after down-/upregulating miR-514a-5p in H295R and SW-13 cells by RT-qPCR. (q) Schematic illustration of C22orf46 3-UTR-Wt/Mut luciferase reporter vectors. (r) The binding ability between C22orf46 3-UTR and miR-514a-5p was detected by dual-luciferase reporter assay in H295R and SW-13 cells. , .

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