lncRNA RHPN1-AS1 Serves as a Sponge for miR-3133 Modulating the Cell Proliferation of Retinoblastoma through JAK2

Figure 1

Silencing of RHPN1-AS1 inhibited the proliferation of RB cell lines and promoted their apoptosis. (a) lncRNA RHPN1-AS1 was upregulated in RB cell lines; , compared with APRE-19; (b) lncRNA RHPN1-AS1 expression was detected after transfection with siRHPN1-AS1 or siNC in Y79 and WERI-Rb1; , compared with siNC; (c) the proliferation activity of Y79 and WERI-Rb1 was detected by CCK-8; and , indicated compared with 0 h; (d) cell proliferation of Y79 and WERI-Rb1 cells was identified by the EDU assay; (e) western blotting was used to detect the expression of apoptosis-related proteins; , compared with siNC; (f) after transfection with siRHPN1-AS1 or siNC, the apoptosis of Y79 and WERI-Rb1 cells was detected by flow cytometry; , compared with siNC.

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