Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway

Figure 3

nsPEF promotes robust liver regeneration. (a) Representative images of HE staining (magnification, ×200) at different time points after liver ablation by nsPEF. (b) IHC staining for F4/80 shows macrophages accumulating at the border zone at 3 days after nsPEF ablation (original magnification, ×40 and ×200, respectively). (c) IHC staining for NIMP-R14 shows neutrophils accumulating at the border zone at 24 hours after nsPEF ablation (original magnification, ×40 and ×200, respectively). (d) Hepatic cell proliferation was detected by IHC staining for Ki67-positive staining cells within the ablated and unablated lobes of the mouse liver at indicated time points after nsPEF ablation (magnification, ×200). (e) Quantification of Ki67-positive cells in the unablated area of ablated lobes and unablated lobes within 14 days in indicated time points after nsPEF ablation. The number of positive cells was counted by the average number of positive cells in 5 random fields of a ×100 microscope. Data were presented as (, , and ).

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