Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway

Figure 4

The liver regeneration induced by nsPEF is related to the activated HGF/c-Met pathway. (a, b) Level of HGF mRNA (a) and protein (b) within liver tissues at the ablated lobe and unablated lobe at indicated time points after nsPEF ablation detected by qRT-PCR and ELISA, respectively. (c, d) Serum level of HGF (c) and VEGF (d) examined by ELISA at indicated time points after nsPEF ablation. (e–g) Mice were administered intraperitoneally with the c-Met inhibitor PHA (30 mg/kg) (PHA) or vehicle (control) daily for 3 successive days after nsPEF ablation. Then, the liver and serum were harvested for the detection of Ki67-positive hepatocytes by IHC staining and serum VEGF level by ELISA. Representative IHC staining results with magnification of ×200 (e), the quantification of the average number of Ki67-positive cells within 5 random fields of ×100 microscopy (f), and serum VEGF level (g) by ELISA on day 3 after ablation. Data were presented as (, , and ).

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