Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway

Figure 6

nsPEF induces a more obvious hepatocyte proliferation at the periablational area in comparison to other unablated areas at ablated lobes. (a) Representative image of IHC staining for Ki67 (original magnification, ×200) shows more obvious hepatocyte proliferation at the periablational zone at 3 days after nsPEF ablation compared to other areas at the ablated lobe. (b) Representative image of IHC staining for cox-2 (original magnification, ×40 and ×200) at the periablational zone at 24 hours after nsPEF ablation. (c, d) Mice were administered intraperitoneally with the cox-2 inhibitor celecoxib (50 mg/kg) or vehicle (control) daily for 3 successive days after nsPEF ablation. Then, livers were harvested for the detection of Ki67-positive staining cells. Representative results of IHC staining with magnification of ×200 at the periablational area of the ablated lobe of mice from the celecoxib group and control group (c) and quantification of Ki67-positive cells within 5 random fields of ×100 microscopy (d). (e) Level of HGF within liver tissues in the unablated area and periablated area within the ablated lobe at indicated time points after nsPEF ablation detected by ELISA, respectively. (f, g) Mice were administered intraperitoneally with the c-Met inhibitor PHA (30 mg/kg) (PHA) or vehicle (control) daily for 3 successive days after nsPEF ablation. Then, livers were harvested for the detection of Ki67-positive staining hepatocytes. Representative results of IHC staining for Ki67 with magnification of ×200 at the periablational area of the ablated lobe of mice from the PHA group and control group (f) and quantification of Ki67-positive cells within 5 random fields of ×100 microscopy (g). Data were presented as (, , and ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)