Cucurbita ficifolia Fruit Extract Induces Tp53/Caspase-Mediated Apoptosis in MCF-7 Breast Cancer Cells

Figure 3

PI staining and TUNEL assay (microscopic) after 24 hs (a–d) and 48 hs (e–h) incubation of MCF-7 treated with 90 μg/mL C. ficifolia chloroform extract with the control. Red fluorescence is due to propodium iodide (PI) (thick arrow) staining observed under green filter. TUNEL-positive cells (thin arrow) are with red fluorescence and blue indicates the nucleus. Observations were done at 200x for PI and 400x for TUNEL magnification.