MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

Figure 2

miR-200a directly regulates CD47 expression in NPC cells. Notes: (a, b) based on a dual-luciferase assay, miR-200a was predicted to directly bind to the 3UTR of CD47 mRNA, and cotransfection of the miR-200a mimic and wild-type promoter resulted in decreased luciferase activity. (c, d) At the mRNA level, CD47 expression was inhibited by the CD47 siRNA in both cell lines. (e, f) At the protein level, CD47 expression was inhibited by the miR-200a mimic in both cell lines. vs. the miR-NC group. vs. the miR-200a inhibitor group. Abbreviations: UTR: untranslated regions; WT: wild type; MUT: mutant-type; CD47: cluster of differentiation 47.

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