PCK1 Deficiency Shortens the Replicative Lifespan of Saccharomyces cerevisiae through Upregulation of PFK1

Figure 4

PFK1 deletion restored PCK1 deficiency strains RLS by inhibited ROS level. (a) The growth curves of BY4742, pck1Δ, and pck1 pfk1Δ yeast cells based on monitoring (A600) at various time points. (b) BY4742, pck1Δ, and pck1Δpfk1Δ yeast cells were diluted (1 : 10) sequentially and cultured on YPD plates. (c) The production of pyruvic acid levels was examined in BY4742, pck1Δ, and pck1Δpfk1Δ strains. Columns, mean of at least three independent experiments; bars, SEM. , , comparison between two groups as indicated. (d) BY4742, pck1Δ, PCK1 OE, and pck1Δpfk1Δ yeast cells were cultured for 12 hours and stained with DCFH-DA. Resuspended cells were subjected to flow cytometric analysis. (e) RLS curves of BY4742, pck1Δ, and pck1Δpfk1Δ strains. Mortality curves were generated from lifespan data, and BY4742 was considered the control. Numbers in parentheses are the median RLS and cell number. (f) Mechanism diagram of PCK1-deficient shortened yeast replication lifespan.

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