LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p

Figure 2

CAR10 promotes the proliferation of cervical cancer cells in vitro and in vivo. We transfected the overexpression plasmid pcDNA-CAR10 and the negative control pcDNA-NC into C33A and HeLa cells and detected the expression level of CAR10 by RT-qPCR (a), , compared with pcDNA-NC. Proliferative capacity of cervical cancer cells C33A and HeLa cells was examined by CCK-8 assay (b, c) and colony formation experiments (d, e), , compared with pcDNA-NC. The mRNA expression levels of cyclin A1, cyclin B1, and CDK2 were detected by RT-qPCR (f, g), , compared with pcDNA-NC. The protein expression levels of cyclin A1, cyclin B1, and CDK2 were detected by western blot (h, i), , compared with pcDNA-NC. (l) The CAR10 stably overexpressing C33A cells were injected subcutaneously into nude mice, and the growth of the neoplasms was monitored in real time, , compared with pcDNA-NC. (m, n) After the nude mice were sacrificed, the neoplasms were removed and the weight was counted. , compared with pcDNA-NC. (o, p) Western blot was used to detect the protein expression levels of cyclin A1, cyclin B1, and CDK2 in the neoplasms, , compared with pcDNA-NC.

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