LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p

Figure 7

CAR10/miR-125b-5p/PDPK1 network regulates the proliferation of cervical cancer cells. miR-125b-5p inhibitors (anti-miR-125b-5p) or PDPK1 siRNA (si-PDPK1) was transfected into C33A and HeLa cells, respectively, (a) the expression level of miR-125b-5p and PDPK1 was detected by RT-qPCR, , compared with Mock. ^Ф, compared with si-NC. (b, c, d) The effects of anti-miR-125b-5p and/or si-PDPK1 on cell proliferation were examined by CCK-8 assay and colony formation assay, , compared with CON. ^Ф, compared with anti-miR-125b-5p + si-NC. (e, f) The effects of anti-miR-125b-5p and/or si-PDPK1 on the expression of cyclin A1, cyclin A2, and CDK2 were detected by RT-qPCR, , compared with CON. ^Ф, compared with anti-miR-125b-5p + si-NC. (g) The effects of anti-miR-125b-5p and/or si-PDPK1 on cell migration were examined by transwell assay, , compared with CON. ^Ф, compared with anti-miR-125b-5p + si-NC. (h, i, j) The effects of CAR10 overexpression and/or si-PDPK1 on cell proliferation were examined by CCK-8 assay and colony formation assay, , compared with CON. ^Ф, compared with CAR10 + si-NC. (k, l) The effects of CAR10 overexpression and/or si-PDPK1 on the expression of cyclin A1, cyclin A2, and CDK2 were detected by RT-qPCR, , compared with CON. ^Ф, compared with CAR10 + si-NC. (m) The effects of CAR10 overexpression and/or si-PDPK1 on cell migration were examined by transwell assay, , compared with CON. ^Ф, compared with CAR10 + si-NC.

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