Research Article
Activation of Inward Rectifier K+ Channel 2.1 by PDGF-BB in Rat Vascular Smooth Muscle Cells through Protein Kinase A
Figure 5
Effects of AC on PDGF-BB-mediated activation of Kir2.1 currents. (a) Original tracings illustrating currents determined in RASMCs. Em was held at -80 mV, and pulse potentials were applied from -150 mV to 0 mV. (b) Arithmetic () of current-voltage relationship curves. (c) Arithmetic () of the current amplitude at -150 mV of Kir2.1 currents in PDGF-BB; PDGF-BB+forskolin. Forskolin (10 μM). vs. PDGF-BB; PDGF-BB+SQ22536. SQ22536 (10 nM). vs. PDGF-BB. (d) The “O” and “C” indicate the opened and closed states of Kir2.1 currents, respectively. (e) The forskolin increased and SQ22536 decreased the NPo of Kir2.1 currents (at +70 mV) by ( vs. PDGF-BB, ) and ( vs. PDGF-BB, ) compared to PDGF-BB alone (). (f) Time course of PDGF-BB action of Kir2.1 currents. Averaged NPo plot (5 s bins) from five repeated perfusions of indicated compounds was normalized to control.
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