Research Article
Preclinical Development of Mahanine-Enriched Fraction from Indian Spice Murraya koenigii for the Management of Cancer: Efficacy, Temperature/pH stability, Pharmacokinetics, Acute and Chronic Toxicity (14-180 Days) Studies
Figure 1
Optimization of the method for the preparation of a mahanine-enriched fraction (MEF) by HPLC. (a) HPLC profiles of the ethanolic extract of the water-extracted M. koenigii leaflet residue and ethyl acetate fraction. Arrow indicates the area of the peak from which percent of mahanine was calculated. (b) HPLC profiles of the ethanol : water (95 : 5) extract of the water-extracted M. koenigii leaflet residue and ethyl acetate fraction. (c–e) HPLC profiles of Methods 3-5 for the preparation of MEFM3-M5 involved in the initial maceration of leaflets with ethanol : water in different ratios. This step is followed by fractionation with ethyl acetate. The mahanine peaks in both steps are indicated by arrows. The HPLC profile of MEF has been reported earlier [14].
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