Evaluation of Zhenwu Decoction Effects on CYP450 Enzymes in Rats Using a Cocktail Method by UPLC-MS/MS

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">CYP450</td><td class="align_center">Forward primer</td><td class="align_center">Reverse primer</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">CYP1A2</td><td class="align_center">CATCTTTGGAGCTGGATTTG</td><td class="align_center">CCATTCAGGAGGTGTCC</td></tr><tr><td class="align_left">CYP2B1</td><td class="align_center">AACCCTTGATGACCGCAGTAAA</td><td class="align_center">TGTGGTACTCCAATAGGGACAAGATC</td></tr><tr><td class="align_left">CYP2C6</td><td class="align_center">TCAGCAGGAAAACGGATGTG</td><td class="align_center">AATCGTGGTCAGGAATAAAAATAACTC</td></tr><tr><td class="align_left">CYP2C7</td><td class="align_center">TGTGAAGAACATCAGCCAATCCT</td><td class="align_center">CACGGTCCTCAATGTTCCTTTT</td></tr><tr><td class="align_left">CYP2C11</td><td class="align_center">GGAGGAACTGAGGAAGAGCA</td><td class="align_center">AATGGAGCATATCACATTGCAG</td></tr><tr><td class="align_left">CYP2D2</td><td class="align_center">GAAGGAGAGCTTTGGAGAGGA</td><td class="align_center">AGAATTGGGATTGCGTTCAG</td></tr><tr><td class="align_left">CYP3A1</td><td class="align_center">TGCCAATCACGGACACAGA</td><td class="align_center">ATCTCTTCCACTCCTCATCCTTAG</td></tr><tr><td class="align_left"><i>β</i>-Actin</td><td class="align_center">GCCCAGAGCAAGACAGGTAT</td><td class="align_center">GGCCATCTCCTGCTCGAAGT</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>The primer for the enzymes and reference genes of rat.</div>

BioMed Research International

tab1

Table 1

Table 1: Evaluation of Zhenwu Decoction Effects on CYP450 Enzymes in Rats Using a Cocktail Method by UPLC-MS/MS 