Evaluation of Zhenwu Decoction Effects on CYP450 Enzymes in Rats Using a Cocktail Method by UPLC-MS/MS

Table 3

Precision of seven probe substrates in rat plasma ().

Probe substrates	Added (ng/mL)	Interday		Intraday
		(ng/mL)	RSD (%)	(ng/mL)	RSD (%)
Phenacetin	2		7.59	2.07±0.13	6.49
	4		5.14	4.28±0.29	6.84
	53		3.79		4.20
	1050		2.12		1.91
Bupropion	2.25		7.49		7.08
	4.5		5.65		5.15
	35		1.90		5.89
	450		3.14		1.39
Diclofenac	30		4.00		7.24
	60		2.64		4.28
	520		2.93		6.48
	6750		5.11		3.58
Amodiaquine	0.6		11.8		11.2
	1.2		8.18		7.15
	10		6.58		6.36
	75		4.84		4.35
Omeprazole	2		7.63		8.77
	4		6.36		7.72
	63.5		5.25		4.79
	1500		4.8		4.07
Dextromethorphan	0.8		5.38		6.03
	1.6		7.09		6.65
	17.9		5.00		5.25
	300		2.12		2.80
Midazolam	6		7.56		6.94
	12		6.20		6.18
	104		4.04		5.29
	1350		4.14		3.70