Downregulation of Long Noncoding RNA LUCAT1 Suppresses the Migration and Invasion of Bladder Cancer by Targeting miR-181c-5p

Figure 4

LUCAT1 downregulation repressed cell proliferation, migration, and invasion and induced cell apoptosis in a miR-181c-5p-dependent manner. (a, b) The expression of LUCAT1 was detected by RT-PCR assay after T24 and UM-UC-3 cells were transfected with si-LUCAT1 or si-NC (, ). T24 and UM-UC-3 cells were transiently transfected with si-NC+inhibitor-NC, si-LUCAT1+inhibitor-NC, si-LUCAT1+inhibitor-miR-181c-5p, and si-NC+inhibitor-miR-181c-5p and thereby submitted to the following assays. (c, d) CCK-8 assay was used to detect cell proliferation ability. (e, f) Flow cytometry assay was carried out to assess cell apoptosis populations. (g, h) Wound healing assay was used to detect cell migration capacity. (i, j) Transwell chambers coated with Matrigel were applied to detect cell invasion (; , compared with si-NC+inhibitor-NC group; ^#, compared with si-LUCAT1+inhibitor-NC group).

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