Research Article
Heme Oxygenase-1 Suppresses Wnt Signaling Pathway in Nonalcoholic Steatohepatitis-Related Liver Fibrosis
Table 1
Primers for quantitative real-time PCR analysis.
| | Gene | Length of production (bp) | Primers |
| | Wnt1 | 81 | F 5-CTCTTTGGCCGAGAGTTCGT-3 | | R 5-TGCCTCGTTGTTGTGAAGGT-3 | | Wnt5a | 80 | F 5-GTTGCTCCGGCCCAGAAG-3 | | R 5-TTGGAAGACATGGCACCTCC-3 | | β-Catenin | 125 | F 5-GTCAGTGCAGGAGGCCG-3 | | R 5-GGCCATGTCCAACTCCATCA-3 | | GSK-3β | 146 | F 5-AGCTCTGATTGGCCACTGTC-3 | | R 5-TAACCAATGAGGAGACGCCG-3 | | NFAT5 | 121 | F 5-GCAGCCTCCAATCTCACACA-3 | | R 5-GCAAGCGTCTGGGGTTATGT-3 | | HO-1 | 82 | F 5-AAGCTTTTGGGGTCCCTAGC-3 | | R 5-GGTGAGGGAACTGTGTCAGG-3 | | TGF-β1 | 111 | F 5-CATTGCAGTGGTTTGGGGTG-3 | | R 5-TCTGGAAAGGACCGTTGTCG-3 | | α-SMA | 156 | F 5-GTACCACCATGTACCCAGGC-3 | | R 5-GCTGGAAGGTAGACAGCGAA-3 | | Col-1 | 198 | F 5-TTCTCCTGGCAAAGACGGAC-3 | | R 5-CGGCCACCATCTTGAGACTT-3 | | Smad4 | 70 | F 5-CTTCCGACAAGTTGGCAGC-3 | | R 5-CCCGAGCCGCTCCGTTA-3 | | GAPDH | 112 | F 5-CAAGAAGGTGGTGAAGCAGG-3 | | R 5-AAAGGTGGAGGAGTGGGTGT-3 |
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