Circular RNA circ_0026359 Enhances Cisplatin Resistance in Gastric Cancer via Targeting miR-1200/POLD4 Pathway

Figure 4

POLD4 was a direct target of miR-1200. (a) Predicted consequential pairing of miR-1200 with the POLD4 3UTR and the mutant consequence of POLD4 3UTR used in the luciferase reporter assay. (b, c) Luciferase reporter assay of SGC-7901/CDDP and MKN-45/CDDP cells cotransfected with miR-1200/miR-NC mimics and luciferase reporter plasmid pmirGLO containing wild-type POLD4 3UTR sequence (hsa_POLD4 3UTR-wt)/mutant POLD4 3UTR sequence (hsa_POLD4 3UTR-mt). (d) mRNA levels of POLD4 in SGC-7901/CDDP and MKN-45/CDDP cells after transfection with miR-1200/miR-NC mimics were examined by RT-qPCR. U6 was detected as control. (e) Protein levels of POLD4 in SGC-7901/CDDP and MKN-45/CDDP cells after transfection with miR-1200/miR-NC mimics were examined by western blot. α-Tubulin was detected as control. ; . ns: no significance.

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