Dexrazoxane Protects Cardiomyocyte from Doxorubicin-Induced Apoptosis by Modulating miR-17-5p

Figure 4

MiR-17-5p directly targets PTEN and regulates its expression. (a) The expression of miR-15-5p, miR-16-5p, miR-17-5p, miR-22-3p, miR-29b-3p, miR-30a-5p, miR-140-5p, miR-155-5p, miR-216a-5p, and miR-208b-3p were detected by qPCR in the Con and DOX groups (). (b) Conservation of the miR-17-5p sequence between mice, rats, and humans is shown. (c) A binding site for miR-17-5p in the 3-UTR of PTEN was analyzed by target prediction programs, and the mutated sequence of the binging site is marked in red. (d) Luciferase activity was assessed in HEK-293A cells 24 h after transfection with the indicated plasmids: miR-17-5p+vectors, miR-17-5p+PTEN 3UTR plasmid, and miR-17-5p+mutated PTEN 3UTR plasmid (). (e,f) The level of PTEN was detected by Western blotting in normal control (NC) and miR-17-5p mimics groups (). (g,h) The expression of PTEN was estimated using Western blotting in normal control of inhibitor (NCi) and miR-17-5p inhibitor groups (). (, ).

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