Determining the Clinical Value and Critical Pathway of GTPBP4 in Lung Adenocarcinoma Using a Bioinformatics Strategy: A Study Based on Datasets from The Cancer Genome Atlas

Figure 8

GTPBP4 regulates LUAD cell proliferation and apoptosis. (a) Knockdown of GTPBP4 in A549 and H1299 cells using si-NC, si-GTPBP4-245, si-GTPBP4-868, and si-GTPBP4-1374 was confirmed by western blotting (left: A549 cells; right: H1299 cells). (b) Viability of A549 and H1299 cells was determined under different concentrations of TP (25, 12.5, 6.25, 3.125, 1.5625, 0.78125, and 0 ng/mL). (c) Proliferation rates of LUAD cells were analyzed using the EdU assay with an IC₅₀ concentration of TP. and . (d) Cell apoptosis was evaluated by Annexin V staining; (), .

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