[Retracted] Rice stripe virus p2 Colocalizes and Interacts with Arabidopsis Cajal Bodies and Its Domains in Plant Cells

Table 1

The sequences, homologous recombination, and restriction sites of PCR primers.

Primer and purpose	Sequence (5→3)^a	Modification
Construction for yeast two-hybrid assay
p2-ADF	CGggatccTGATGGCATTACTCCTTTTC	BamHI
p2-ADR	CCGctcgagTCACATTAGAATAGGACACT	XhoI
Atcoilin-BDF	GgaattcATGGAGGAAGAGAAGGTGAGGTG	EcoRI
Atcoilin-1-BDR	CGggatccTCACCTTTCCTCAACCTCAAT	BamHI
Atcoilin-2-BDF	CCcatatgATGGGCCTCAGATTCGTCCTGGTG	NdeI
Atcoilin-2-BDR	CGggatccTCAAGAGGCTTCATCTGTTCC	BamHI
Atcoilin-3-BDF	CCcatatgATGCTTGATAGTGAACCTCTTG	NdeI
Atcoilin-BDR	CGggatccTCAAATCTCTTTCTGAGATC	BamHI
Construction for entry vector pDONR221
p2-GF	ggggacaagtttgtacaaaaaagcaggcttcATGGCATTACTCC TTTTC	Homologous recombination
p2-GR	ggggaccactttgtacaagaaagctgggtcCATTAGAATAGGACACT	Homologous recombination
Atcoilin-GF	ggggacaagtttgtacaaaaaagcaggcttcATGGAGGAAGAGAAGGTG	Homologous recombination
Atcoilin-1-GR	ggggaccactttgtacaagaaagctgggtcCCTTTCCTCAACCTCAAT	Homologous recombination
Atcoilin-2-GF	ggggacaagtttgtacaaaaaagcaggcttcATGGGCCTCAGATTCGTCCT	Homologous recombination
Atcoilin-2-GR	ggggaccactttgtacaagaaagctgggtcAGAGGCTTCATCTGTTCC	Homologous recombination
Atcoilin-3-GF	ggggacaagtttgtacaaaaaagcaggcttcATGCTTGATAGTGAACCTCT	Homologous recombination
Atcoilin-GR	ggggaccactttgtacaagaaagctgggtcAATCTCTTTCTGAGATC	Homologous recombination

^aThe letters in lower case indicate homologous recombination sequence or a restriction enzyme site.