Resveratrol Modifies Lipid Composition of Two Cancer Cell Lines

Table 1

Saturated lipid profile of MCF-7 and MDA-MB-231 cells after resveratrol treatment.

FA ID	Lipid name	R.T.	MCF-7		MDA-MB-231
			Control	RESV	Control	RESV
			(μg/μL)		(μg/μL)
C12 : 0	Lauric acid	9.774	0.212^a	0.304^a	0.015^a	0.013^a
C13 : 0	Tridecanoic acid	11.740	0.023^a	0.011^a	ND	ND
C14 : 0	Myristic acid	14.676	0.158 ± 0.011	0.184 ± 0.096	0.081 ± 0	0.106 ± 0.014
C15 : 0	Pentadecanoic acid	18.323	0.035 ± 0.002	0.036 ± 0.020	0.013 ± 0.009	0.018 ± 0.012
C16 : 0	Palmitic acid	23.714	0.745 ± 0.100	0.946 ± 0.538	0.814 ± 0.235	0.926 ± 0.184
C17 : 0	Margaric acid	28.721	0.036 ± 0.016	0.044 ± 0.030	0.049 ± 0.036	0.064 ± 0.045
C18 : 0	Stearic acid	35.921	0.583 ± 0.285	0.846 ± 0.611	0.997 ± 0.587	1.125 ± 0.572
C20 : 0	Eicosanoic acid	48.923	0.012 ± 0.001	0.014 ± 0.003	0.015 ± 0	0.019 ± 0
C21 : 0	Heneicosanoic acid	56.060	0.004^a	ND	ND	ND
C22 : 0	Docosanoic acid	63.220	0.011 ± 0.007	0.013 ± 0.003	0.010 ± 0.007	0.015 ± 0.003
C23 : 0	Tricosanoic acid	70.271	0.003^a	ND	ND	ND
C24 : 0	Lignoceric acid	77.220	0.009 ± 0.006	0.010 ± 0.001	0.023 ± 0.002	0.043 ± 0.010
C26 : 0	Hexacosanoic acid	82.293	ND	ND	0.015 ± 0.002	0.023 ± 0
Total saturated lipid content			1.831	2.408	2.032	2.352

FA ID: fatty-acid identification; RESV: resveratrol; R.T.: retention time; ND: not determined. ^aFatty acid detected in only one independent experiment. MCF-7 and MDA-MB-231 (1 × 10⁷) cells after resveratrol (80 and 200 μM, respectively) or DMSO 0.1% (control) treatment for 24 h were subjected to lipid extraction. Lipid extracts were analyzed by GC/MS. Results are expressed as mean ± S.E.M., n = 2.