Resveratrol Modifies Lipid Composition of Two Cancer Cell Lines
Table 2
Unsaturated lipid profile of MCF-7 and MDA-MB-231 cells after resveratrol treatment.
FA ID
Lipid name
R.T.
MCF-7
MDA-MB-231
Control
RESV
Control
RESV
(μg/μL)
(μg/μL)
C16 : 1n9
7-Hexadecenoic acid
21.867
0.027 ± 0
0.040 ± 0.025
0.112a
0.095a
C16 : 1n7
Palmitoleic acid
22.104
0.223 ± 0.133
0.252 ± 0.061
0.093 ± 0.005
0.131 ± 0.030
C17 : 1n7
Cis-10-heptadecanoic acid
27.261
0.027 ± 0.014
0.026 ± 0.01
0.023 ± 0.005
0.038 ± 0.013
C18 : 2n6
Linoleic acid
32.871
0.036 ± 0.012
0.038 ± 0.002
0.038 ± 0.012
0.056 ± 0.001
C18 : 1n9c
Oleic acid
33.820
0.542 ± 0.076
0.831 ± 0.478
0.460 ± 0.014
0.718 ± 0.206
C18 : 1n9t
Elaidic acid
34.094
0.419 ± 0.168
0.334 ± 0.002
0.162 ± 0.005
0.234 ± 0.036
C20 : 4n6
Arachidonic acid
43.802
0.073 ± 0.014
0.102 ± 0.064
0.123 ± 0.071
0.235 ± 0.159
C20 : 5n3
Eicosapentaenoic acid (EPA)
44.181
0.011 ± 0.001
0.019 ± 0.016
0.040 ± 0.020
0.060 ± 0.036
C20 : 3n6
8, 11, 14-Eicosatrienoic acid
45.067
0.016 ± 0.001
0.025 ± 0.020
0.022 ± 0.008
0.053 ± 0.031
C20 : 2n6
11, 14-Eicosadienoic acid
47.228
0.005a
0.007 ± 0.004
0.007a
0.012 ± 0.003
C20 : 1n9
Cis-11-Eicosaenoic acid
48.739
0.038 ± 0.013
0.042 ± 0.030
0.013a
0.011 ± 0.007
C20 : 1
49.254
0.036 ± 0.025
0.027 ± 0.012
ND
0.002a
C22 : 6n3
4, 7, 10, 13, 16, 19-Docosahexaenoic acid (DHA)
57.155
0.052 ± 0.010
0.086 ± 0.057
0.084 ± 0.049
0.160 ± 0.108
C22 : 1n9
Erucic acid
61.184
0.014 ± 0.010
0.014 ± 0.004
0.010 ± 0.004
0.008 ± 0.004
C24 : 1n9
Nervonic acid
75.325
0.034 ± 0.019
0.038 ± 0.013
0.012 ± 0.006
0.019 ± 0.002
Total unsaturated lipid content
1.553
1.881
1.199
1.832
FA ID: fatty-acid identification; RESV: resveratrol; R.T.: retention time; ND: Not determined. aFatty acid detected in only one independent experiment. MCF-7 and MDA-MB-231 (1 × 107) cells after resveratrol (80 and 200 μM, respectively) or DMSO 0.1% (control) treatment for 24 h were subjected to lipid extraction. Lipid extracts were analyzed by GC/MS. Results are expressed as mean ± S.E.M., n = 2.
