Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45

Figure 3

Genome organization of the R4 phage. The 45 kb double-stranded DNA (black parallel lines) contains 69 coding genes, each represented by arrows, annotated as indicated. The modular genome organization, characteristic for Staphylococcal siphophages is highlighted by colors, indicating the coding regions belonging to one of the six functional modules. The 1455 bp long gene orf27, coding for the endolysin (amidase), marked by a black asterisk, was cloned into the 2.9 kb pRSET A vector. The rightmost black image represents the result of XhoI-HindIII probe digestion of orf27-pRSET A construct (lane “A”) and pRSET A control (lane “B”) on 1% agarose gel. Excised 1.5 kb orf27 band is marked by a white asterisk. (Lane “L”: DNA ladder.)