Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen

Table 1

The primers used in this study.

Primers	Abreaction	Sequences (5′ ⟶ 3′)	Position	Length (bp)
DPOL-1	DPOL-F1	ATGACATTCTTTAATCCATAT	3129–3149	1143
	DPOL-R1	TCCGACACCCAGCCTATACAAT	4250–4271
DPOL-2	DPOL-F2	TGCCCACATCTATGAGTTTC	4109–4128	1152
	DPOL-R2	CTCTAGACGAAAGGACATTGT	5239–5259
DPOL-3	DPOL-F3	TGACGGTGCATGTAAACAAGA	4933–4953	1222
	DPOL-R3	TTTTACATAACAATGACACT	6135–6154
qPCR	qPCMV-F	GCGTAGAACTCGTTAGAA	5542–5559	188
	qPCMV-R	GCCAATTATGATGTCAATGATC	5709–5729
	qPCMV-P	TCCGTTCCGTATCACTTCGTCG	5666–5687	—

The location sequence region of primers and probe was calculated based on GenBank number AF268039 (strain B6), the DPOL gene located at positions 3,129 to 6,152 of strain B6 (GenBank number AF268039).