Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen

Table 2

Reproducibility test of intra-assay and interassay for the real-time qPCR method.

Copies/μl	Intra-assay			Interassay
	CT	SD	CV (%)	CT	SD	CV (%)
5.19 × 10^8	11.32	0.10	0.92	11.40	0.15	1.32
5.19 × 10^7	14.65	0.12	0.80	14.70	0.13	0.91
5.19 × 10^6	17.79	0.13	0.74	17.83	0.15	0.82
5.19 × 10^5	21.26	0.12	0.57	21.32	0.15	0.68
5.19 × 10^4	24.19	0.16	0.67	24.27	0.16	0.65
5.19 × 10^3	27.26	0.16	0.58	27.35	0.21	0.77
5.19 × 10^2	30.07	0.32	1.07	30.20	0.54	1.80
5.19 × 10^1	33.64	0.51	1.51	34.08	0.76	2.24