Research Article

Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen

Table 3

Detection porcine semen by conventional PCR and real-time qPCR.

NumberPositive samplesCopies/μl
Conventional PCRqPCRBoth#Aonly#B

15247.35 × 104 and 2.49 × 1056.18 × 102 and 2.13 × 102

#APorcine semen were tested positive by conventional PCR and real-time qPCR. #BPorcine semen were tested with conventional PCR negative and real-time qPCR positive.