Screening of T Cell-Related Long Noncoding RNA-MicroRNA-mRNA Regulatory Networks in Non-Small-Cell Lung Cancer

Figure 7

ceRNA network of (a) CD8(+) T cell-related DEGs and (b) memory-activated CD4(+) T cell-related DEGs and chemical small molecule-target network analysis of (c) CD8(+) T cell-related DEGs and (d) memory-activated CD4(+) T cell-related DEGs. The lncRNA-miRNA interaction of the immune-related genes was predicted by using the relevant database (miRWalk3.0, TargetScan, MiRDB, and MirTarBase), with a threshold . The prediction module of DIANA-LncBase v.2 database was used to predict the miRNAs that might be related to lncRNAs, which were related with CD8(+) T cell- and memory-activated CD4(+) T cells. Based on the lncRNA-miRNA and miRNA-target interaction edges, the lncRNA-miRNA-mRNA network was constructed utilizing the Cytoscape software. The red diamond represents the lncRNAs, the green triangle represents the miRNAs, and the red circle represents the mRNAs. The “Carcinoma, Non-Small-Cell Lung” was used as the keyword in the Comparative Toxicogenomics Database to search for lung cancer-related genes and chemicals. Besides, the overlapping genes that were associated with lung cancer and belonged to the T cells related genes in the ceRNA network were used to screen the chemical-target edges. Furthermore, the small chemical molecule-target network of the T cells was generated by using the Cytoscape software. The CD8(+) T cell-related DEG’s group involved 6 mRNAs and 16 small chemical molecule drugs. The memory-activated CD4(+) T cell-related DEG’s group involved 1 mRNAs and 2 small molecule drugs. Red represents mRNA, and green represents small molecule drugs.

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