Serum miR-375 Levels Are Closely Related to Disease Progression from HBV Infection to HBV-Related Hepatocellular Carcinoma

Figure 4

The effect of miR-375 on the proliferation of HCC cells. We performed the CCK-8 assay in HepG2 cells, HepG2.2.15 cells, and HepAD38 cells at 24 h, 48 h, and 72 h after transfecting the cells with miR-375 mimics. HepG2.2.15 and HepAD38 cells were positive for HBsAg. However, HepG2 cells were negative for HBsAg. The proliferation rates of miR-375-transfected cells were lower than those of nontransfected cells (transfected vs. nontransfected HepG2.2.15 cells, transfected vs. nontransfected HepAD38 cells, and transfected vs. nontransfected HepG2 cells). The proliferation rates of HepG2.2.15 cells and HepAD38 cells were lower than those of HepG2 cells (transfected HepG2.2.15 cells vs. transfected HepG2 cells, transfected HepAD38 cells vs. transfected HepG2 cells, and nontransfected HepG2.2.15 cells vs. nontransfected HepG2 cells).