Orosomucoid 1 Attenuates Doxorubicin-Induced Oxidative Stress and Apoptosis in Cardiomyocytes via Nrf2 Signaling

Figure 2

Downregulation of ORM1 and upregulation of oxidative stress and apoptosis in the DOX-induced cardiomyopathy model in vitro and in vivo. (a) Differentially expressed candidate proteins in control and DOX-treated mice analyzed using LC/MS technology. Low expression is depicted in green, and high expression is depicted in red. (b) mRNA levels of ORM1, Nrf2, and HO-1 in the hearts of mice in the DOX-treated groups (). (c, d) Western blot analysis of ORM1, Nrf2, HO-1, 4-HNE, and cleaved caspase-3 (). (e, f) Immunohistochemical staining images of ORM1- and 4-HNE-stained heart sections from control and DOX-treated mice (). (g) mRNA levels of ORM1, Nrf2, and HO-1 in DOX-treated H9c2 cells (10 μM and 24 h) and the control group (). (h, i) Western blot analysis of ORM1, Nrf2, HO-1, 4-HNE, and cleaved caspase-3 in DOX-treated H9c2 cells (10 μM and 24 h) and the control group (). Data are expressed as the mean . and .

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