Orosomucoid 1 Attenuates Doxorubicin-Induced Oxidative Stress and Apoptosis in Cardiomyocytes via Nrf2 Signaling

<div>ORM1 reduces doxorubicin- (DOX-) induced oxidative stress and apoptosis in H9c2 cells. (a, b) Western blot analysis of ORM1, Nrf2, HO-1, 4-HNE, and cleaved caspase-3. (c) Cell survival rate analysis using the Cell Counting Kit 8 (CCK-8). Cell survival rate is expressed as the optical density (OD) value (% control). (d) Cellular malondialdehyde (MDA) content. (e, f) Fluorescence image (red fluorescence) of reactive oxygen species (ROS) measured using dichlorodihydrofluorescein diacetate (DCFH-DA). (g, h) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining images with calculated apoptosis indices. 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BioMed Research International

fig3

Figure 3

Figure 3: Orosomucoid 1 Attenuates Doxorubicin-Induced Oxidative Stress and Apoptosis in Cardiomyocytes via Nrf2 Signaling 