Effect of Carvacrol, TRP Channels Modulator, on Cardiac Electrical Activity

Figure 4

CAR effects on cardiac electrical activity of rabbit heart recorded by optical mapping using the fluorescent dye di-4-ANBDQBS. Representative optical maps of activation time (AT), conduction velocity (CV), conduction vector maps (C-vector), and OAPD maps (OAPD20, OAPD50, and OAPD90) obtained under control conditions and at 10, 30, 100, 300, and 1000 μM concentrations of CAR. Endocardial pacing was set at 300 ms. The number near the isochrones shows the activation times in AT maps (in ms). The conduction velocity is shown in CV maps (in m/s). AP duration maps are shown for OAPD20, OAPD50, and OAPD90 (in ms). The interval between isochrones is 2 ms for the AT and 0.2 m/s for the CV. The AT was calculated as the time interval from stimulus to 50% depolarization. OAPD20, OAPD50, and OAPD90 were calculated at 20%, 50%, and 90% of repolarization, respectively, from the AT. Note: 1000 μM CAR was applied after the washout period with Tyrode solution without the drug. The corresponding scale bars are given in the left and at the bottom.